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Nucleic Acids Research, 1984, Vol. 12, No. 7 3333-3342
© 1984

MOLECULAR BIOLOGY

The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli

Shunsuke Ishii, Makoto Ihara, Toshio Maekawa*, Yoshikazu Nakamura*, Hisao Uchida* and Fumio Imamoto+

Laboratory of Molecular Genetics, Riken (Institute of Physical and Chemical Research) Hirosawa, Wako-shi, Saitama 351 *Institute of Medical Science, University of Tokyo P.O. Takanawa, Tokyo 108, Japan

+To whom correspondence should be addressed

Received January 3, 1984. Revised March 14, 1984. Accepted March 14, 1984.

The nucleotide sequence of the promoter-proximal portion of the nusA operon including the genes for tRNAf2Mct, a 15 kilodalton protein and the initial portion of the nusA gene has been determined previously (1). Here, we report the sequence for the entire nusA gene and its flanking region. The open reading frame, consisting of 1,482 nucleotides, was identified as that of the nusA protein on the basis of agreement of the amino acid sequence deduced from the DNA sequence with the N-terminal sequence of the purified nusA protein. The molecular weight of 54,417 daltons calculated for the 494 amino acid polypeptide is significantly lower than that determined previously by SDS polyacrylamide gel analysis. The nusA gene is immediately followed by another open reading frame encoding a polypeptide of at least 22 amino acids, which was identified as the initial portion of the infB structural gene. In the spacer region of 24 base pairs between the nusA and infB structural genes there is no significant DNA sequence that fits the canonical transcriptional termination signal or promoter sequence. We suggest, therefore, that the genes for tRNAf2Met, a 15 kilodalton protein, the nusA protein and IF2{alpha}, aligned in this order, are co-transcribed.


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