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Nucleic Acids Research, 1984, Vol. 12, No. 8 3435-3444
© 1984

MOLECULAR BIOLOGY

A colorimetric method for DNA hybridization

Manfred Renz and Christina Kurz

European Molecular Biology Laboratory Postfach 10.2209, 6900 Heidelberg, FRG

Received March 12, 1984. Accepted April 6, 1984.

A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences complementary to the probe can be visualized directly without the need of tedious cytochemical sandwich methods. This procedure will detect target sequences, a few kilobases long, in the 1- to 5-pg range.


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