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Nucleic Acids Research, 1984, Vol. 12, No. 8 3611-3618
© 1984

MOLECULAR BIOLOGY

Nucleotide sequence of two rasH related-genes isolated from the yeast Saccharomyces cerevisiae

R. Dhar1, A. Nieto1, R. Koller1, D. Defeo-Jones2 and E.M. Scolnick2

1Laboratory of Molecular Virology, National Cancer Institute Bethesda, MD 20205, USA 2Department of Virus and Cell Biology, Merck Sharp and Dohme Research Laboratories West Point, PA 19486, USA

Received February 24, 1984. Revised March 19, 1984. Accepted March 19, 1984.

A complete nucleotide sequence of two ras-related yeast genes (c-rasSC-1 and c-rasSC-2 ) isolated from the yeast strain Saccharomyces cerevisiae is reported. They encode predicted polypeptides of 40,000 and 41,000 daltons, respectively. The N-terminal 170 amino acids from both genes show extensive amino acid homology to other ras genes from vertebrates, whereas their C-termini have diverged. These genes should be useful in the elucidation of a normal biological function of ras-related genes in a simple system like yeast.


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