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Nucleic Acids Research, 1984, Vol. 12, No. 9 3791-3806
© 1984

MOLECULAR BIOLOGY

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli

Michael A. Boss, John H. Kenten, Clive R. Wood and J.Spencer Emtage

Celltech Ltd, 244–250 Bath Road, Slough SL1 4DY, Berks, UK

Received March 5, 1984. Revised April 16, 1984. Accepted April 16, 1984.

Genes for a murine µ heavy chain and a {lambda} light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosoroe binding site. Induction of transcription from the trp promoter results in accumulation of both light and heavy chain polypeptides in appropriate host strains. Both proteins were found as Insoluble products. Following extraction and purification of the immunoglobulin containing fractions, antigen binding activity was recovered. The activity demonstrates essentially the same properties as the antibody from the hybridoma from which the genes were cloned.


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