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Nucleic Acids Research, 1984, Vol. 12, No. 9 3807-3819
© 1984

MOLECULAR BIOLOGY

Molecular amplification and purification of the E. colt recC gene product

Ian D. Hickson1, Karen E. Atkinson2, Linda Hutton, Alan E. Tomkinson and Peter T. Emmerson

Department of Biochemistry, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK

Received February 13, 1984. Revised April 4, 1984. Accepted April 16, 1984.

The level of recC gene expression has been analysed using Mud.(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing conditions. The recC gene product has been amplified by harnessing the gene to the phage lambda leftward promoter in a plasmid. From cells harbouring this plasmid, RecC protein, which represented approximately 6% of the total cellular protein, was purified to eleotrophoretic homogeneity.

1Present address: Department of Clinical Oncology, University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, UK

2Present address: Department of Molecular Biology and Biochemistry, Harvard University, Cambridge, Massachusetts, USA


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