Nucleic Acids Research, 1984, Vol. 12, No. 9 3873-3893
© 1984
MOLECULAR BIOLOGY |
Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin
Department of Biological Sciences, University of Pittsburgh Pittsburgh, PA 15260, USA
Received December 14, 1984. Revised April 5, 1984. Accepted April 5, 1984.
Recombinant plasmids that direct synthesis of rat preproinsulin under the direction of the SV40 early promoter have been used to probe the mechanism of initiation of translation. Insertion of an upstream AUG triplet that was out-of-frame with respect to the coding sequence for preproinsulin red iced the yield of proinsulin, in keeping with the predictions of the scanning model. The extent to which an upstream AUG codon interfered depended on sequences surrounding the AUG triplet; with two constructs (p255/20 and C2) the 5'-proximal AUG codon constituted an absolute barrier: there was no initiation at the downstream start site for preproinsulin. With two other constructs (p255/9, p255/21), however, proinsulin was made despite the presence of an upstream, out-of-frame AUG codon in a favorable context for initiation. In those cases the reading frame set by the first AUG triplet was short, terminating before the start of the preproinsulin coding sequence. The interpretation that ribosomes initiate at the first AUG, terminate, and then reinitiate at the AUG that directly precedes the preproinsulin coding sequence was tested by introducing a point mutation that eliminated the terminator codon: the resulting mutant made no proinsulin.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Ma and S. S. Taylor A Molecular Switch for Targeting between Endoplasmic Reticulum (ER) and Mitochondria: CONVERSION OF A MITOCHONDRIA-TARGETING ELEMENT INTO AN ER-TARGETING SIGNAL IN DAKAP1 J. Biol. Chem., April 25, 2008; 283(17): 11743 - 11751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Montero, L. M. Gonzalez, M. Rodriguez, Y. Oksov, M. J. Blackman, and C. A. Lobo A Conserved Subtilisin Protease Identified in Babesia divergens Merozoites J. Biol. Chem., November 24, 2006; 281(47): 35717 - 35726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Moniaux, W. M. Junker, A. P. Singh, A. M. Jones, and S. K. Batra Characterization of Human Mucin MUC17: COMPLETE CODING SEQUENCE AND ORGANIZATION J. Biol. Chem., August 18, 2006; 281(33): 23676 - 23685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. de Mayolo, M. Lisby, N. Erdeniz, T. Thybo, U. H. Mortensen, and R. Rothstein Multiple start codons and phosphorylation result in discrete Rad52 protein species. Nucleic Acids Res., January 1, 2006; 34(9): 2587 - 2597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Sun, D.-Y. Jin, R. M. Camire, and D. W. Stafford Vitamin K epoxide reductase significantly improves carboxylation in a cell line overexpressing factor X Blood, December 1, 2005; 106(12): 3811 - 3815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamasaki, S. Hashiguchi, Y. Ito, Z. Kato, K. Nakanishi, T. Nakashima, and K. Sugimura Human Anti-Human IL-18 Antibody Recognizing the IL-18-Binding Site 3 with IL-18 Signaling Blocking Activity J. Biochem., October 1, 2005; 138(4): 433 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kato, C. Cortese-Grogan, S. A. Moyer, F. Sugahara, T. Sakaguchi, T. Kubota, N. Otsuki, M. Kohase, M. Tashiro, and Y. Nagai Characterization of the Amino Acid Residues of Sendai Virus C Protein That Are Critically Involved in Its Interferon Antagonism and RNA Synthesis Down-Regulation J. Virol., July 15, 2004; 78(14): 7443 - 7454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Oshima, T. Nakamura, S. Namiki, E. Okada, K. Tsuchiya, R. Okamoto, M. Yamazaki, T. Yokota, M. Aida, Y. Yamaguchi, et al. Interferon Regulatory Factor 1 (IRF-1) and IRF-2 Distinctively Up-Regulate Gene Expression and Production of Interleukin-7 in Human Intestinal Epithelial Cells Mol. Cell. Biol., July 15, 2004; 24(14): 6298 - 6310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Rajendran, A. C. Nye, J. Frasor, R. D. Balsara, P. G. V. Martini, and B. S. Katzenellenbogen Regulation of Nuclear Receptor Transcriptional Activity by a Novel DEAD Box RNA Helicase (DP97) J. Biol. Chem., February 7, 2003; 278(7): 4628 - 4638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kato, Y. Ohnishi, M. Hishiyama, M. Kohase, S. Saito, M. Tashiro, and Y. Nagai The Amino-Terminal Half of Sendai Virus C Protein Is Not Responsible for either Counteracting the Antiviral Action of Interferons or Down-Regulating Viral RNA Synthesis J. Virol., June 14, 2002; 76(14): 7114 - 7124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kato, Y. Ohnishi, M. Kohase, S. Saito, M. Tashiro, and Y. Nagai Y2, the Smallest of the Sendai Virus C Proteins, Is Fully Capable of both Counteracting the Antiviral Action of Interferons and Inhibiting Viral RNA Synthesis J. Virol., April 15, 2001; 75(8): 3802 - 3810. [Abstract] [Full Text]
|
|
|
|
|
|
S. Sei, Q.-e. Yang, D. O'Neill, K. Yoshimura, K. Nagashima, and H. Mitsuya Identification of a Key Target Sequence To Block Human Immunodeficiency Virus Type 1 Replication within the gag-pol Transframe Domain J. Virol., May 15, 2000; 74(10): 4621 - 4633. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. J. Lynch, E. Touma, W. Niforatos, K. L. Kage, E. C. Burgard, T. van Biesen, E. A. Kowaluk, and M. F. Jarvis Molecular and Functional Characterization of Human P2X2 Receptors Mol. Pharmacol., December 1, 1999; 56(6): 1171 - 1181. [Abstract] [Full Text]
|
|
|
|
|
|
S. Svensson, P. Stromberg, and J.-O. Hoog A Novel Subtype of Class II Alcohol Dehydrogenase in Rodents. UNIQUE PRO47 and SER182 MODULATES HYDRIDE TRANSFER IN THE MOUSE ENZYME J. Biol. Chem., October 15, 1999; 274(42): 29712 - 29719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Burgard, W. Niforatos, T. van Biesen, K. J. Lynch, E. Touma, R. E. Metzger, E. A. Kowaluk, and M. F. Jarvis P2X Receptor-Mediated Ionic Currents in Dorsal Root Ganglion Neurons J Neurophysiol, September 1, 1999; 82(3): 1590 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. V. Pilipenko, E. G. Viktorova, E. V. Khitrina, S. V. Maslova, N. Jarousse, M. Brahic, and V. I. Agol Distinct Attenuation Phenotypes Caused by Mutations in the Translational Starting Window of Theiler's Murine Encephalomyelitis Virus J. Virol., April 1, 1999; 73(4): 3190 - 3196. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. E. G. McCarthy Posttranscriptional Control of Gene Expression in Yeast Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1492 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Finlin and D. A. Andres Rem Is a New Member of the Rad- and Gem/Kir Ras-related GTP-binding Protein Family Repressed by Lipopolysaccharide Stimulation J. Biol. Chem., August 29, 1997; 272(35): 21982 - 21988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Furukawa, H. Soejima, N. Niikawa, H. Shiku, and K. Furukawa Genomic Organization and Chromosomal Assignment of the Human beta 1,4-N-Acetylgalactosaminyltransferase Gene. IDENTIFICATION OF MULTIPLE TRANSCRIPTION UNITS J. Biol. Chem., August 23, 1996; 271(34): 20836 - 20844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Walker, C. Girardet, and J. F. Habener Alternative Exon Splicing Controls a Translational Switch from Activator to Repressor Isoforms of Transcription Factor CREB during Spermatogenesis J. Biol. Chem., August 16, 1996; 271(33): 20219 - 21050. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Opalenik, J. T. Shin, J. N. Wehby, V. K. Mahesh, and J. A. Thompson The HIV-1 TAT Protein Induces the Expression and Extracellular Appearance of Acidic Fibroblast Growth Factor J. Biol. Chem., July 21, 1995; 270(29): 17457 - 17467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Zysow, G. E. Lindahl, D. P. Wade, B. L. Knight, and R. M. Lawn C/T Polymorphism in the 5' Untranslated Region of the Apolipoprotein(a) Gene Introduces an Upstream ATG and Reduces In Vitro Translation Arterioscler. Thromb. Vasc. Biol., January 1, 1995; 15(1): 58 - 64. [Abstract] [Full Text]
|
|
|
|
 |
|
 K Y Miller, J Wu, and B L Miller StuA is required for cell pattern formation in Aspergillus. Genes & Dev., September 1, 1992; 6(9): 1770 - 1782. [Abstract] [PDF]
|
|
|
|
|
|
J F Kukowska-Latallo, R D Larsen, R P Nair, and J B Lowe A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase. Genes & Dev., August 1, 1990; 4(8): 1288 - 1303. [Abstract] [PDF]
|
|
|
|
|
|
W S Moye-Rowley, K D Harshman, and C S Parker Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins. Genes & Dev., March 1, 1989; 3(3): 283 - 292. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Malhotra, K. Hart, H. Klamut, N. Thomas, S. Bodrug, A. Burghes, M Bobrow, P. Harper, M. Thompson, P. Ray, et al. Frame-shift deletions in patients with Duchenne and Becker muscular dystrophy Science, November 4, 1988; 242(4879): 755 - 759. [Abstract] [PDF]
|
|
|
|
|
|
F. Gizard, B. Lavallee, F. DeWitte, and D. W. Hum A Novel Zinc Finger Protein TReP-132 Interacts with CBP/p300 to Regulate Human CYP11A1 Gene Expression J. Biol. Chem., August 31, 2001; 276(36): 33881 - 33892. [Abstract] [Full Text] [PDF]
|
|