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Nucleic Acids Research, 1984, Vol. 12, No. 9 4011-4017
© 1984

MOLECULAR BIOLOGY

Oligonudeotide mutagenesis of the lacPUV5 promoter

Lianna M. Munson, Wlodzimierz Mandecki*, M.H. Caruthers* and William S. Reznikoff

Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin Madison, WI 53706 *Department of Chemistry, University of Colorado Boulder, CO 80309, USA

Received January 3, 1984. Revised April 9, 1984. Accepted April 9, 1984.

Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions –13, –14, and –15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.


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