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Nucleic Acids Research, 1985, Vol. 13, No. 1 115-129
© 1985

Articles

DNaseI sensitivity of the rat albumin and {alpha}-fetoprotein genes

Lisa Kunnath and Joseph Locker1,*

Departments of Pathology and Biochemistry, University of Chicago Chicago, IL 60637, USA

*To whom correspondence should be sent

Received April 19, 1984. Revised November 20, 1984. Accepted November 20, 1984.

We have analyzed the DNaseI sensitivity of chromatin from the rat albumin and {alpha}-fetoprotein genes in the fetal liver (which synthesizes albumin and {alpha}-fetoprotein), adult liver (which synthesizes albumin), fetal yolk sac (which synthesizes {alpha}-fetoprotein), and adult kidney (which synthesizes neither). Active genes were much more sensitive than their kidney counterparts, and the adult liver {alpha}-fetoprotein and fetal yolk sac albumin genes showed intermediate levels of sensitivity. Sensitivity was analyzed as a function of the extent of DNaseI digestion. Rate constants were calculated for the degradation of individual DNA hybridization bands and normalized to the intrinsic rate constants of the same bands degraded in purified DNA. This enabled us to eliminate the inconsistencies that otherwise result from comparing chromatin sensitivity of different DNA sequences, or chromatin sensitivity in different nuclear environments.

1Present address: School of Medicine, Department of Pathology, University of Pittsburgh, 777A Scaife Hall, Pittsburgh, PA 15261, USA


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J K Liu, Y Bergman, and K S Zaret
The mouse albumin promoter and a distal upstream site are simultaneously DNase I hypersensitive in liver chromatin and bind similar liver-abundant factors in vitro.
Genes & Dev., May 1, 1988; 2(5): 528 - 541.
[Abstract] [PDF]


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