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Nucleic Acids Research, 1985, Vol. 13, No. 1 131-140
© 1985

Articles

Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168

John S. Miles and John R. Guest*

Department of Microbiology, University of Sheffield Sheffield S10 2TN, UK

*To whom correspondence should be addressed

Received November 20, 1984. Accepted December 13, 1984.

The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein identified from expression in Escherichia coli maxicells. There is no sequence homology between the B. subtilis and E. coli fumarases. 0ver1a potential promoter sequences have been identified for {sigma}28, {sigma}37 and {sigma}55 RNA polymerase holoenzymes. The DNA fragment also contains the proximal part of the gerA locus, responsible for L-alanine-sensitive spore germination.


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