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Nucleic Acids Research, 1985, Vol. 13, No. 1 195-205
© 1985

Articles

Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes

Philippe Mazodier, Pascale Cossart*, Evelyne Giraud and Francis Gasser

Unite de Physiologie Cellulaire, Département de Biochimie et Génétique Moléculaire, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France *Unité de Biochimie Cellulaire, Département de Biochimie et Génétique Moléculaire, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France

Received October 19, 1984. Revised December 10, 1984. Accepted December 10, 1984.

The DNA sequence of the region located downstream from the kanamycin resistance gene of Tn5 up to the right inverted repeat IS5OR has been determined. This completes the determination of the sequence of Tn5 which is 5818 bp long. The 2.7 Kb central region contains three resistance genes: the kanamycin-neomycin resistance gene, a gene coding for resistance to CL990 an antimitotic-antibiotic compound of the bleomycin family and a third gene that confers streptomycin resistance in some bacterial species but is cryptic in E. coli. A Tn5* mutant able to express streptomycin resistance in E. coli was isolated. With this mutant, it was demonstrated that in E. coli the expression of the three resistance genes is coordinated in a single operon.


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