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Nucleic Acids Research, 1985, Vol. 13, No. 1 239-249
© 1985

Articles

Primary structure of the soybean nodulin-23 gene and potential regulatory elements in the 5'-flanking regions of nodulin and leghemoglobin genes

Vincent P. Mauro, Truyen Nguyen, Panagiotis Katinakis and Pal S. Verma*

Plant Molecular Biology Laboratory, Department of Biology, McGill University 1205 Docteur Penfield Avenue, Montreal, Quebec, H3A 1B1, Canada

*To whom correspondence should be addressed

Received September 27, 1984. Accepted December 3, 1984.

The nodulin-23 gene of soybean is one of the most abundantly transcribed genes induced during symbiosis with Rhizobium. Using a plasmid (pNod25) from a nodule cDNA library, we have isolated the nodulin gene from a soybean genomic library. Nucleotide sequence analysis of the cDNA and of the genomic clone indicated that the coding region of this gene is 669 bp long and is interrupted by a single intron of about 53ø bp. The deduced protein sequence suggests that nodulin-23 may have a signal sequence. The 5'-flanking sequence of two other nodulin genes, nodulin-24 encoding for a mambrane polypeptide and one of the leghanoglobin genes (LbC3), were obtained. Comparison of these sequences revealed three conserved eg ions, one of which, an octanucleotide (GTTTCCCT), has 1øø% homology. The conserved sequences are arranged in a unique fashion and have a spatial organization with respect to order and position, which nay suggest a potential regulatory role in controlling the expression of nodulin and leghanoglobin genes during symbiosis.


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