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Nucleic Acids Research, 1985, Vol. 13, No. 1 45-57
© 1985

Articles

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

Tomas Kempe, Wesley I. Sundquist, Flora Chow and Shiu-Lok Hu

Molecular Genetics, Inc. 10320 Bren Road East, Minnetonka, MN 55343, USA

Received October 9, 1984. Revised November 23, 1984. Accepted December 7, 1984.

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.


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