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Nucleic Acids Research, 1985, Vol. 13, No. 10 3651-3666
© 1985

Articles

Improved rapid phosphotriester synthesis of oligodeoxyribonueleotides using oxygen-nucleoplillic cataiysts

V. A. Efimov*, O. G. Chakhmakhcheva and Yu. A. Ovchinnikov

Shemyakin Institute of Bioorganic Chemistry USSR, Academy of Sciences Ul. Miklukho-Maklaya, 16/10 117871 GSP, Moscow, USSR

*To whom reprint request should be sent

Received March 14, 1985. Revised May 1, 1985. Accepted May 1, 1985.

The use of different condensing and phosphorylating agents in conjunction with oxygen-nucleophilic catalysts, such as 4-substituted derivatives of pyridine N-oxide and quinoline N-oxide, leads to a dramatic increase of the rate of the phosphotriester bond formation and minimizes the amount of by-products caused by the modification of heterocyclic bases. The application of these catalysts to the solid-phase oligonucleotide synthesis allows to reduce the time needed for the performance of one elongation cycle on a polymer support to 10 min.


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