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Nucleic Acids Research, 1985, Vol. 13, No. 11 4133-4141
© 1985

Articles

New DNA polymorphism: evidence for a low salt, left-handed form of poly(dG-m5dC)

Burt G. Feuerstein1,+, Laurence J. Marton1,2, Max A. Keniry3, Donald L. Wade3 and Richard H. Shafer3,*

1Brain Tumor Research Center, School of Pharmacy, University of California San Francisco, CA 94143, USA 2Department of Laboratory Medicine, School of Medicine, School of Pharmacy, University of California San Francisco, CA 94143, USA 3Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco, CA 94143, USA

*To whom correspondence should be addressed

Received April 4, 1985. Revised May 10, 1985. Accepted May 10, 1985.

Spectroscopic studies on solutions of poly(dG-m5 dC) over a wide range of salt concentration are presented. Low salt solutions ([Na+]) < 2 mM) of poly(dG-m5dC) produce circular dichroism (CD) spectra typical of the left-handed, Z form at high salt ([Na+] = 1.75 M). Solutions of poly(dG-m5dC) at intermediate salt concentrations, e.g., 142 mM, yield CD spectra characteristic of the right-handed, B conformation. 31P NMR spectra of the low salt form of poly(dG-m5dC) reveal two well separated peaks, split by 1.4 ppm, consistent with a dinucleotide repeat. Kinetic studies show that the transition from the low salt form to the right-handed B form is slow, as expected for a major conformational change. These results suggest that the Z conformation in poly(dG-m5 dC) can be stabilized at very low salt as well as at high salt

+Present address: Department of Pediatrics, School of Medicine, University of California, San Francisco, Ca 94143


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