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Nucleic Acids Research, 1985, Vol. 13, No. 11 4191-4202
© 1985

Articles

Characterisation of P. falciparum antigenic determinants isolated from a genomic expression library by differential antibody screening

Gordon Langsley, Artur Scherf*, Odile Mercereau-Puijalon, Michael Koenen+,, Bernard Kahane, Denise Mattei, Micheline Guillotte, Lise Sibilli, Ian Garner, Benno Müller-Hill* and Luiz Pereira da Silva

*Institut für Genetik der Universität zu Köln 5 Köln 41, FRG Institut Pasteur 28 rue du Dr Roux, 75724 Paris, Cedex 15, France

Received February 22, 1985. Revised April 12, 1985. Accepted April 12, 1985.

A genomic expression library of P.falciparum has been differentially screened with a number of immune sera. The response of 9 clones to the various sera is presented, together with the DNA sequence encoding the epitopes. All but one clone are extremely A+T rich and unlike the other P.falciparum epitopes described, are not composed of amino acid repeats. One clone, which responds specifically with a protective serum, has been analysed in detail. The epitope is carried on a 160kd antigen which is transcribed from a single gene to give a protein expressed in all of the erythrocytic forms. DNA sequence of this clone reveals it to have more than one open reading frame, only one of which is transcribed in the blood stages. The possible significance of the other open readings frames is discussed

+Present address: ZMBH Heidelberg, Im Neuenheimer Feld 364, FRG


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