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Nucleic Acids Research, 1985, Vol. 13, No. 11 4203-4224
© 1985

Articles

Small nuclear RNAs in the ciliate Tetrahymena

Nina Pedersen, Per Hellung-Larsen and Jan Engberg

Department of Biochemistry B, University of Copenhagen Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark

Received January 26, 1985. Accepted April 10, 1985.

We have isolated and partially characterized a family of small nuclear RNAs (snRNAs) from three different species of the protozoan Tetrahymena. We find six distinct snRNAs ranging in size from 100 to 250 nucleotides. The two largest snRNAs, as well as an abundant, heterogenous group of smaller snRNAs are found in the nucleolar RNA fraction. None of the snRNAs are transcription products of the ribosomal RNA gene or its flanking regions, as shown by hybridization tests. The snRNAs are metabolically stable as determined by pulse/ chase experiments and several of them contain a number of modified nuclotides. The snRNAs from Tetra-hymena all have slightly different sizes from mammalian snRNAs. The cap structure of the snRNAs from Tetrahymena differs from that of the snRNAs from mammalian cells, but has not yet been fully characterized. The relative amount of snRNAs to total RNA is less in Tetrahymena (>0.1%) than in mammalian cells (0.2%)


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