Skip Navigation

This Article
Right arrow Print PDF (1390K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (88)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Laird, P. W.
Right arrow Articles by Borst, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laird, P. W.
Right arrow Articles by Borst, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 12 4253-4266
© 1985

Articles

Mature mRNAs of Trypanosoma brucei possess a 5' cap acquired by discontinuous RNA synthesis

Peter W. Laird, Jan M. Kooter, Nel Loosbroek and Piet Borst

Department of Molecular Biology, Netherlands Cancer Institute H8 Antoni van Leeuwenhoek Huis, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

Received April 29, 1985. Accepted May 31, 1985.

Mature mRNAs of Trypanosoma brucei have a common 5' terminal sequence of 35 nucleotldes. This is acquired by an unknown mechanism from the 5' end of a separately transcribed precursor RNA of about 140 nt called the mini-exon-derived RNA or medRNA. We have Investigated the nature of the 5' ends of mature mRNAs and of the medRNA by chemical decapping and enzymic recapping. We infer that a 51 cap Is present on both of these RNAs and conclude that the mini-exon-derived RNA donates its 51 cap along with the mini-exon sequence to the pre-mRNA. Using nuclear run-on experiments we show that medRNA synthesis is much more sensitive to a-amanitin than 5S RNA synthesis and only slightly less sensitive than tubulin gene transcription. This result, together with the presence of a cap at the 5' end of the medRNA indicates that the mini-exon is transcribed by an RNA polymer-ase II type enzyme. Our experiments also confirm the existence of a second minor medRNA of about 125 nt and show the presence of other small capped RNAs possibly analogous to the small nuclear RNAs of other organisms


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
A. Gunzl, T. Bruderer, G. Laufer, B. Schimanski, L.-C. Tu, H.-M. Chung, P.-T. Lee, and M. G.-S. Lee
RNA Polymerase I Transcribes Procyclin Genes and Variant Surface Glycoprotein Gene Expression Sites in Trypanosoma brucei
Eukaryot. Cell, June 1, 2003; 2(3): 542 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Gilinger and V. Bellofatto
Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms
Nucleic Acids Res., April 1, 2001; 29(7): 1556 - 1564.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Lamontagne and B. Papadopoulou
Developmental Regulation of Spliced Leader RNA Gene in Leishmania donovani Amastigotes Is Mediated by Specific Polyadenylation
J. Biol. Chem., March 5, 1999; 274(10): 6602 - 6609.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. R. Sturm, M. C. Yu, and D. A. Campbell
Transcription Termination and 3'-End Processing of the Spliced Leader RNA in Kinetoplastids
Mol. Cell. Biol., February 1, 1999; 19(2): 1595 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Lee and L. Van der Ploeg
Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei
Science, December 14, 1990; 250(4987): 1583 - 1587.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.