Skip Navigation

This Article
Right arrow Print PDF (941K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (67)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Montandon, P.-E.
Right arrow Articles by Stutz, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Montandon, P.-E.
Right arrow Articles by Stutz, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 12 4299-4310
© 1985

Articles

Streptomycin-resistance of Euglena gracilis chloroplasts: identification of a point mutation in the 16S rRNA gene in an invariant position

Paul-Etienne Montandon, Paul Nicolas1, Peter Schümann and Erhard Stutz*,

1Département de Biologie générale et appliquée, Université de Lyon I 43 Bvd du 11 Novembre 1918, F-69622 Villeurbanne Cédex, France Laboratoire de Biochimie végétale, Université de Neuchâtel Chantemerle 18, CH-2000 Neuchâtel, Switzerland

*To whom correspondence should be addressed

Received April 15, 1985. Revised May 23, 1985. Accepted May 23, 1985.

We sequenced the chloroplast 16S rRNA gene of two Euglena gracilis mutants which contain streptomycin-resistant chloroplasts (Smr 139.12/4 and Smr 139.20/2). These mutants are known to contain a single intact rrn operon per circular chloroplast genome. Nucleotlde sequence comparison between a 16S rRNA gene of wild type Euglena gracilis, strain Z, with streptomycin-sensitive chloroplasts, and the 16S rRNA gene of both Smr-strains reveals a single base change (C to T) at position 876. This position is equivalent to the invariant position 912 of the E.coli 16S rRNA gene. The analogous position is also conserved in all chloroplast small subunit RNA genes from lower and higher plants sequenced so far. Light dependent protein synthesis with purified chloroplasts from reptomycin-resistant cells is not inhibited by streptomycin. Based on the results reported here we postulate linkage between the observed point mutation on the 16S rRNA gene and streptomycin-resistance of chloroplast 70S ribosomes


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
D. Criswell, V. L. Tobiason, J. S. Lodmell, and D. S. Samuels
Mutations Conferring Aminoglycoside and Spectinomycin Resistance in Borrelia burgdorferi
Antimicrob. Agents Chemother., February 1, 2006; 50(2): 445 - 452.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Matsuoka, K. Takahama, and T. Ogawa
Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rps12 gene that allows selection of mutants free from drug resistance markers
Microbiology, August 1, 2001; 147(8): 2077 - 2087.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. A. McConkey, M. J. Rogers, and T. F. McCutchan
Inhibition of Plasmodium falciparum Protein Synthesis. TARGETING THE PLASTID-LIKE ORGANELLE WITH THIOSTREPTON
J. Biol. Chem., January 24, 1997; 272(4): 2046 - 2049.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.