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Nucleic Acids Research, 1985, Vol. 13, No. 12 4527-4538
© 1985

Articles

Sequence of a pseudogene in the legumin gene family of pea (Pisum sativum L.)

David Bown, Mark Levasseur, Ronald R.D. Croy, Donald Boulter and John A. Gatehouse

Department of Botany, Durham University South Road, Durham DH1 3LE, UK

Received April 4, 1985. Accepted May 29, 1985.

A second legumin gene, denoted {psi}Leg D, has been located on the pea genomic clone {lambda}Leg 1, approx.1.3 Kbases 3' of Leg A, in the same orientation. The complete sequence of {psi}Leg D shows that it is a pseudogene, having two stop codons near the 5' end of its predicted coding sequence, as well as deletions and frame shift errors when compared to Leg A. No transcripts from this gene could be detected in developing pea seeds. Leg A and {psi}Leg D are homologous over their coding sequences, and partially homologous in the intron sequences and the immediate 5' flanking sequences. Other flanking sequences of the two genes show no significant homology, apart from the presence of polyadenylation signals 3' to both coding sequences. The introns in the two genes occur in corresponding positions in the sequences, but a deletion in {psi}Leg D affects the 3' boundary of IVS-2. Hybridisation of {psi}Leg D to pea genomic DNA suggests that it does not represent a hitherto undetected subfamily of legumin genes


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