Nucleic Acids Research, 1985, Vol. 13, No. 12 4539-4555
© 1985
Articles |
Nitrogen fixation specific regulatory genes of Klebsiella pneumoniae and Rhizobium meliloti share homology with the general nitrogen regulatory gene ntrC of K. pneumoniae
1Department of Molecular Biology, Massachusetts General Hospital Boston, MA 02114 2Department of Genetics, Harvard Medical School Boston, MA 02115 3Department of Cellular and Developmental Biology, Harvard University Cambridge, MA 02138 4Department of Chemistry, Massachusetts Institute of Technology Cambridge, MA 02139, USA
*To whom correspondence should be addressed
Received April 1, 1985. Revised May 29, 1985. Accepted May 29, 1985.
We have determined the complete nucleotide sequences of three functionally related nitrogen assimilation regulatory genes from Klebsiella pneumoniae and Rhizobium meliloti. These genes are:1) The K.pneumoniae general nitrogen assimilation regulatory gene ntrC (formerly called glnG), 2) the K.cpneumoniae nif-specific regulatory gene nifA, and 3) an R. meliloti nif-specific regulatory gene that appears to be functionally analogous to the K. pneumoniae nifA gene. In addition to the DNA sequence data, gel-purified K. pneumoniae nifA protein was used to determine the amino acid composition of the nifA protein. The K.pneumoniae ntrC and nifA genes code for proteins of 52,259 and 53,319 d respectively. The R. meliloti nifA gene codes for a 59,968 d protein. A central region within each polypeptide, consisting of approximately 200 amino acids, is between 52% and 58% conserved among the three proteins. Neither the amino termini nor the carboxy termini show any conserved sequences. Together with data that shows that the three regulatory proteins aotivate promoters that share a common consensus sequence in the -10 (5'-TTGCA-3') and -23 (5'-CTGG-3') regions, the sequenoe data presented here suggest a common evolutionary origin for the three regulatory genes
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Martinez, J. M. Palacios, J. Imperial, and T. Ruiz-Argueso Symbiotic Autoregulation of nifA Expression in Rhizobium leguminosarum bv. viciae J. Bacteriol., October 1, 2004; 186(19): 6586 - 6594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Katayama, H. Suzuki, T. Koyanagi, and H. Kumagai Cloning and Random Mutagenesis of the Erwinia herbicola tyrR Gene for High-Level Expression of Tyrosine Phenol-Lyase Appl. Envir. Microbiol., November 1, 2000; 66(11): 4764 - 4771. [Abstract] [Full Text]
|
|
|
|
|
|
S. Zhao, Q. Zhu, and R. L. Somerville The sigma 70 Transcription Factor TyrR Has Zinc-Stimulated Phosphatase Activity That Is Inhibited by ATP and Tyrosine J. Bacteriol., February 15, 2000; 182(4): 1053 - 1061. [Abstract] [Full Text]
|
|
|
|
|
|
J. Li, L. Passaglia, I. Rombel, D. Yan, and S. Kustu Mutations Affecting Motifs of Unknown Function in the Central Domain of Nitrogen Regulatory Protein C J. Bacteriol., September 1, 1999; 181(17): 5443 - 5454. [Abstract] [Full Text]
|
|
|
|
|
|
A. D. Laurie and G. Lloyd-Jones The phn Genes of Burkholderia sp. Strain RP007 Constitute a Divergent Gene Cluster for Polycyclic Aromatic Hydrocarbon Catabolism J. Bacteriol., January 15, 1999; 181(2): 531 - 540. [Abstract] [Full Text]
|
|
|
|
 |
|
 C Freiberg, X Perret, W J Broughton, and A Rosenthal Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable "sequenase": a beginning. Genome Res., July 1, 1996; 6(7): 590 - 600. [Abstract] [PDF]
|
|
|
|
 |
|
 S B Sharma and E R Signer Temporal and spatial regulation of the symbiotic genes of Rhizobium meliloti in planta revealed by transposon Tn5-gusA. Genes & Dev., March 1, 1990; 4(3): 344 - 356. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Popham, D Szeto, J Keener, and S Kustu Function of a bacterial activator protein that binds to transcriptional enhancers Science, February 3, 1989; 243(4891): 629 - 635. [Abstract] [PDF]
|
|