Skip Navigation

This Article
Right arrow Print PDF (2137K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Daubas, P.
Right arrow Articles by Buckingham, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Daubas, P.
Right arrow Articles by Buckingham, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 13 4623-4643
© 1985

Articles

A comparison between mammalian and avian fast skeletal muscle alkali myosin light chain genes: regulatory implications

Philippe Daubas, Benoit Robert, Ian Garner and Margaret Buckingham

Department of Molecular Biology, Institut Pasteur 25 rue du docteur Roux, 75724 Paris Cedex 15, France

Received May 17, 1985. Accepted June 4, 1985.

A single locus in the mouse, rat and chicken encodes both alkali myosin light chains, MLC1F and MLC3F. This gene has two distinct promoters and gives rise to two different primary transcripts, which are processed by alternative and different modes of splicing to form MLC1F and MLC3F mBNAs. The MLC1F/MLC3F gene is very similar between mouse, rat and chicken, in terms of its overall structure, the length and location of the introns, and the splice site consensus sequences. Nucleotide sequences of coding regions are very conserved but 3' and 5' non coding regions of the mRNAs have diverged . In the MLC1F promoter regions, several blocks of nucleotides are highly conserved (more than 70 % homology), especially a sequence of about 70 nucleotides, located between positions -80 and -150 relative to the Cap site. Conserved blocks of homology are also found in the MLC3F promoter regions, although the common sequences are shorter. The presence of such highly conserved nucleotide sequences in the 5' flanking regions suggests that these sequences are functionally important in initiation of transcription and regulation of expression of this complex gene. Primer extension experiments indicate multiple cap sites for MLC3F mRNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
M. J. Donoghue, B. L. Patton, J. R. Sanes, and J. P. Merlie
An axial gradient of transgene methylation in murine skeletal muscle: genomic imprint of rostrocaudal position
Development, December 1, 1992; 116(4): 1101 - 1112.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
M Donoghue, H Ernst, B Wentworth, B Nadal-Ginard, and N Rosenthal
A muscle-specific enhancer is located at the 3' end of the myosin light-chain 1/3 gene locus.
Genes & Dev., December 1, 1988; 2(12b): 1779 - 1790.
[Abstract] [PDF]

Home page
 ScienceHome page
S. Malhotra, K. Hart, H. Klamut, N. Thomas, S. Bodrug, A. Burghes, M Bobrow, P. Harper, M. Thompson, P. Ray, et al.
Frame-shift deletions in patients with Duchenne and Becker muscular dystrophy
Science, November 4, 1988; 242(4879): 755 - 759.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.