Skip Navigation

This Article
Right arrow Print PDF (1702K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (204)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Harshman, K. D.
Right arrow Articles by Dervan, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harshman, K. D.
Right arrow Articles by Dervan, P. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 13 4825-4835
© 1985

Articles

Molecular recognition of B-DNA by Hoechst 33258+

Keith D. Harshman and Peter B. Dervan*

Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, CA 91125, USA

*To whom correspondence should be addressed

Received March 18, 1985. Revised May 27, 1985. Accepted May 27, 1985.

The binding sites of Hoechst 33258, netropsin and distamycin on three DNA restriction fragments from plasmid pBR322 were compared by footprinting with methidiumpropyl EDTA·Fe(II) [ MPE·Fe(II)]. Hoechst, netropsin and distamycin share common binding sites that are five ± one bp in size and rich in A·T DNA base pairs. The five base pair protection patterns for Hoechst may result from a central three base pair recognition site bound by two bisbenzimidazole NHs forming a bridge on the floor of the minor groove between adjacent adenine N3 and thymine O2 atoms on opposite helix strands. Hydrophobic interaction of the flanking phenol and N-methylpiperazine rings would afford a steric blockade of one additional base pair on each side.

+Contribution no. 7162 from Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
R. F. Martin, S. Broadhurst, M. E. Reum, C. J. Squire, G. R. Clark, P. N. Lobachevsky, J. M. White, C. Clark, D. Sy, M. Spotheim-Maurizot, et al.
In Vitro Studies with Methylproamine: A Potent New Radioprotector
Cancer Res., February 1, 2004; 64(3): 1067 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. R. Stauffer, T. L. Howard, T. Nyun, and S. M. Hollenberg
CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression
J. Cell Sci., January 7, 2001; 114(13): 2383 - 2393.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Gavathiotis, G. J. Sharman, and M. S. Searle
Sequence-dependent variation in DNA minor groove width dictates orientational preference of Hoechst 33258 in A-tract recognition: solution NMR structure of the 2:1 complex with d(CTTTTGCAAAAG)2
Nucleic Acids Res., February 1, 2000; 28(3): 728 - 735.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. van de Sande, N. Ramsing, M. Germann, W Elhorst, B. Kalisch, E von Kitzing, R. Pon, R. Clegg, and T. Jovin
Parallel stranded DNA
Science, July 29, 1988; 241(4865): 551 - 557.
[Abstract] [PDF]

Home page
 ScienceHome page
P. Dervan
Design of sequence-specific DNA-binding molecules
Science, April 25, 1986; 232(4749): 464 - 471.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.