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Nucleic Acids Research, 1985, Vol. 13, No. 13 4871-4888
© 1985

Articles

Isolation and sequence of cDNA clones coding for a member of the family of high mobility group proteins (HMG-T) in trout and analysis of HMG-T-mRNA's in trout tissues

B.T. Pentecost1, J.M. Wright2 and G.H. Dixon*

Department of Medical Biochemistry, Health Sciences Centre, University of Calgary 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada

*To whom correspondence should be addressed

Received February 25, 1985. Revised May 27, 1985. Accepted May 27, 1985.

A specific oligonucleotide has been used to isolate a cDNA prepared from the mRNA for a trout High Mobility Group (HMG) protein closely related to trout HMG-T and bovine HMG 1 and 2 proteins. The sequence isolated more closely resembles bovine HMG-1 than the previously sequenced HMG-T protein in regions corresponding to the N terminal half of the protein. Northern blot analysis at low stringency indicated that 2 related sequences are expressed in total trout testis mRNA. Southern blots of total trout DNA indicate that several different forms of the homologous sequence are present in the trout genome and an estimate of copy number by dot-blot shows 4 HMG-T genes per trout sperm DNA equivalent. Analysis of mRNA from several trout tissues, including testis, liver and kidney indicates that expression of genes for histones and the larger HMG proteins in trout is not closely coupled.

1Present address: NIEHS, M.D. 1301, P.O. Box 12233, Research Triangle Park, NC 27709, USA

2Albert Heritage Foundation for Medical Research Post-Doctoral Fellow


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