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Nucleic Acids Research, 1985, Vol. 13, No. 13 4953-4969
© 1985

Articles

Alkali-labile structures linked to the 5' ends of Bacillus subtilis short DNA chains

Hachiro Yasuda* and Tuneko Okazaki+

Institute of Molecular Biology, Faculty of Science, Nagoya University Nagoya 4 Japan

+To whom correspondence should be addressed

Received April 22, 1985. Revised June 4, 1985. Accepted June 4, 1985.

Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to tri- ribonucleotide stretches were isolated at the 5' end of the short, DNA chains. A structure for the novel oligonucleotide is proposed to be p5' X3' pp5' rN, where X represents unidentified nucleoside with a peculiar property. Possible metabolic relationship between these molecules and primer RNA has been discussed.

*Present address: Organic Materials Laboratory, Fujitsu Laboratories Ltd., Atsugi 1, Wakamiya, Atsugi 243-01, Japan


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