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Nucleic Acids Research, 1985, Vol. 13, No. 14 5145-5155
© 1985

Articles

Are there insertions in the ribosomal DNA of vertebrates?

Klaus Lohmann* and Karin Kraus

Institut Für Biologie I (Zoologie), Universität Freiburg Albertstrasse 21a, 7800 Freiburg, FRG

Received April 12, 1985. Revised June 6, 1985. Accepted June 6, 1985.

We have analysed the Eco RI restriction pattern of rDNA of the newt Triturus vulgaris and of some other amphibian species by Southern blotting and hybridization with nick-translated Xenopus rDNA prepared from the recombinant plasmids pXlrl 1 and pXlr 12 (21). After hybridization with rl 1, the 28S coding fragments become visible in two bands, a prominent one of 5.3 kb and a weak band of 5.9 kb representing about 8 % of the 28S genes. The evidence obtained so far by additional digestions with Bam HI and Bgl II indicates that in this species and in Triturus helveticus the coding regions of the 5.9 kb fragments are interrupted by an insertion 0.6 kb in length located in a 1.6 kb Bgl II fragment at the 3' end of the Eco RI fragment, which we believe to be the first described in a vertebrate.

*Present address: Max Planck Institute für Züchtungsforschung, 5000 Koln-Vogelsang, FRG.


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