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Nucleic Acids Research, 1985, Vol. 13, No. 14 5203-5213
© 1985

Articles

Trans acting regulation of ß globin gene expression in erythroleukemia (K562) cells

Katherine Young, Maryann Donovan-Peluso, Rosemarie Cubbon and Arthur Bank*

Columbia University, College of Physicians and Surgeons, Department of Medicine, and Department of Human Genetics and Development New York, USA

*To whom correspondence should be addressed at Columbia University, Hammer Health Sciences Center, Room 1602, 701 West 168th Street, New York, NY 10032, USA

Received January 14, 1985. Revised June 24, 1985. Accepted June 24, 1985.

K562 cells are induced by hemin to produce {gamma} and {gamma} globin but not ß globin, although the ß globin gene is intact, and when isolated is expressed in a transient expression assay (1, 2). We have previously shown that an {varepsilon} globin gene transferred into K562 cells is expressed and inducible (3). In this paper, we report the stable transfer of a sickle or ßS globin gene into K562 cells. Thirty-six differentransformed lines were tested; 24 of 36 lines contained an intact ßS globin gene. However, using S1 nuclease, Dot blot, and Northern blotting analyses, none of these lines showed ß globin mRNA expression. These results indicate that trans acting factors are responsible for the lack of expression of the ß globin gene in K562 cells.


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