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Nucleic Acids Research, 1985, Vol. 13, No. 14 5249-5254
© 1985

Articles

Nuclear magnetic resonance observation of the triple interaction between A9 and AU12 in yeast tRNAPhe

B.S. Choi* and A.G. Redfield+

Department of Biochemistry, Brandeis University Waltham, MA 02254, USA

+To whom correspondence should be addressed. Also at the Physics Department and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University

Received May 21, 1985. Accepted June 24, 1985.

The nuclear Overhauser effect (NOE) was used to identify one of the amino proton resonances of base A23 in interaction with A9. These bases form a triple with U12 in the D stem of yeast tRNAPhe. The identification was verified by finding an NOE from this amino proton to the C8 proton of A9, as determined by comparisons of NOE's in a native and a C8-deuterated sample.

*Present Address: Department of chemistry, Massachusetts Institute of Technology, MA, U.S.A.


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