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Nucleic Acids Research, 1985, Vol. 13, No. 14 5389-5402
© 1985

Articles

Adenovirus E3-early promoter: sequences required for activation by E1A

Daniel L. Weeks1 and Nicholas C. Jones

Department of Biological Sciences, Purdue University West Lafayette, IN 47907, USA

Received December 27, 1984. Revised May 31, 1985. Accepted May 31, 1985.

To identify sequences within the adenoviru-5 E3 promoter necessary for E1A trans-activation, a series of promoter deletion mutants were constructed and analysed. A region between positions –82 and –105 was shown to be critical both for E1A induced expression as well as uninduced expression. The importance of this region was confirmed by constructing hybrid promoters consisting of E3 and Herpes simplex virus thymidine kinase sequences. The E1A insensitive tk promoter could be converted to an E1A sensitive promoter by replacing sequences upstream of position –79 with the corresponding region of the E3 promoter. This critical region of the E3 promoter contains a sequence 5' AGATGACTA3' which is also present in important upstream regions of the E2A and E4 promoters.

1Present address: Department of Biochemistry and Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA


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