Nucleic Acids Research, 1985, Vol. 13, No. 15 5657-5669
© 1985
Articles |
The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonastransposon Tn501
Department of Biochemistry, University of Bristol, University Walk Bristol BS8 1TD, UK
Received June 21, 1985. Accepted July 22, 1985.
The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr-111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.
*Present address: Public Health Research Institution of the City of New York Inc., 455 First Avenue, New York, N.Y. 10016, USA
+Present address: Biozentrum der Universität Besel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
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