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Nucleic Acids Research, 1985, Vol. 13, No. 15 5707-5716
© 1985

Articles

Evidence for sequence-specific conformational changes in DNA from the melting temperatures of DNA phosphorothioate derivatives

J. William Suggs and Donna A. Taylor

Department of Chemistry, Brown University Providence, RI 02912, USA

Received March 11, 1985. Revised July 1, 1985. Accepted July 1, 1985.

Analogs of alternating purine-pyrimidine DNA polymers such as poly(dA-dT)-poly(dA-dT) can be made with phosphorothioate groups in the DNA backbone. A phosphorothioate diester at the 5'-purine-pyrimidine-3' step causes a significant lowering of the polymer's melting temperature compared to a phosphorothioate diester at the 5'-pyrimidine-purine-3' step. This may occur because sulfur substitution increases anionic charge density in the DNA minor groove and 5'-purine-pyrimidine-3' steps narrow the minor groove. The ability to modulate charge density in the DNA backbone via sulfur substitution should prove useful in studies of sequence-dependent conformational changes in DNA.


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