Skip Navigation

This Article
Right arrow Print PDF (3777K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Deutscher, S. L.
Right arrow Articles by Wold, W. S.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deutscher, S. L.
Right arrow Articles by Wold, W. S.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 16 5771-5788
© 1985

Articles

Novel deletion mutants that enhance a distant upstream 5' splice in the E3 transcription unit of adenovirus 2

Susan L. Deutscher1, Bheem M. Bhat, Michael H. Pursley, Christos Cladaras and William S.M. Wold*

Institute for Molecular Virology St. Louis, MO 63110, USA 1The Edward A. Doisy Department of Biochemistry, Saint Louis University School of Medicine St. Louis, MO 63110, USA

*To whom correspondence should be addressed

Received May 29, 1985. Accepted July 24, 1985.

Region E3 of adenovirus is a "complex" transcription unit: i.e.different mRNAs and proteins arise by differential RNA 3' end selection and differential splicing of the primary transcript. We are using viable virus mutants to understand the controls that dictate the specificity and efficiency of the RNA processing signals. We describe a novel class of deletion mutations that enhance a natural 5' splice site located {small tilde}740 nucleotides (nt)upstream. In particular, deletions within nt 1691-2044in the E3 transcription unit result in a 5-fold enhancement of the 5' splice site at nt 951 (as reflected in steady-state mRNA). The effect is specific, because the deletions do not affect the 5' splice site at nt 372, and because deletions within nt 2044-2214 do not affect either the 951 or the 372 5' splice sites. As a consequence of the enhanced splicing at the 951 5' site, synthesis of the major E3 mRNA and the major E3 protein(gp19K) are dramatically reduced. At least one of the natural 3' splice sites, located at nt 2157, is the recipient of the enhanced splicing at the 951 5' splice site. We conclude that sequences located within nt 1691-2044 affect (probably in cis) splicing at the 951 5' splice site. We speculate that nt 1691-2044 includes a splicing control region which functions to suppress splicing at the 951 5' splice site.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
A. E. Tollefson, A. Scaria, B. Ying, and W. S. M. Wold
Mutations within the ADP (E3-11.6K) Protein Alter Processing and Localization of ADP and the Kinetics of Cell Lysis of Adenovirus-Infected Cells
J. Virol., July 15, 2003; 77(14): 7764 - 7778.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.