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Nucleic Acids Research, 1985, Vol. 13, No. 16 5869-5882
© 1985

Articles

Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor

Yasuji Furutani, Mitsue Notake, Michiko Yamayoshi, Jun-ichi Yamagishi, Hideki Nomura, Mayumi Ohue, Ryuji Furuta, Toshikazu Fukui, Masaaki Yamada and Shinichi Nakamura

Research Laboratories, Dainippon Pharmaceutical Co. Ltd. Enoki-cho 33-94, Suita/Osaka 564, Japan

Received June 21, 1985. Accepted August 2, 1985.

DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino add residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.


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