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Nucleic Acids Research, 1985, Vol. 13, No. 16 5919-5926
© 1985

Articles

A convenient technique to compare the efficiency of promoters in Escherichia coli

D. Vidal-Ingigliardi and O. Raibaud

Unité de Génétique Moléculaire, Institut Pasteur 28 rue du Dr.Roux, 75724 Paris Cedex 15, France

Received June 4, 1985. Revised July 30, 1985. Accepted July 30, 1985.

We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four wellknown promoters: lacZp, trp, tac, {lambda}PR and three malT activated promoters: malPp, malKp and malEp.


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