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Nucleic Acids Research, 1985, Vol. 13, No. 16 5995-6014
© 1985

Articles

The pathway of E. coli RNA polymerase-promoter complex formation as visualized by footprinting

Bernd Hofer1, Dieter Müller and Hubert Köster

Institut für Organische Chemie und Biochemie der Universität Hamburg Martin-Luther-King-Platz 6, D-2000 Hamburg 13, FRG

Received February 19, 1985. Revised July 23, 1985.

The pathway of E.coli RNA polymerase - promoter complex formation was probed by charcterization of low temperature intermediated at the major coat protein promoter of phage fd DNA. Three different complexes could be distinguished. One of them represents the active ‘open’ complex, the other two have to be regarded as ‘closed’. The promoter contacts of the lower temperature complexes. Increase in temperature led to extension of contacts into the downstream direction, while the upstream border of the ‘open’ complex contacts were extended beyond the start site of transcription.

1Present address: Biosyntech, Stresemannstrasse 268–280, D-2000 Hamburg 50, FRG


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