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Nucleic Acids Research, 1985, Vol. 13, No. 17 6273-6282
© 1985

Articles

Nucleotide sequence of Candida pelliculosa ß-glucosidase gene

Chie Kohchi and Akio toh–e*

Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo-cho Higashi-Hiroshima 724, Japan

* To whom correspondence should be addressed

Received June 25, 1985. Revised August 9, 1985. Accepted August 9, 1985.

The nucleotide sequence of the DNA fragment containing the ß-glucosidase gene of Candida pelliculosa was determined. Analysis of the sequence revealed three open reading frames which could encode 65,825, and 412 amino acid residues. The presence of the second frame was found to be sufficient for the expression of the ß-glucosidase gene in a heterologous host Saccharomyces cerevisiae. Putative protein encoded by this gene had hydrophobic amino acids, resembling a signal peptide, at its N-terminal region and 19 potential glycosylation sites. Codon usage of Candida genes had the similar pattern shown in S.cerevisiae. Codon bias of the ß-glucosidase gene of Candida was relatively low, compared with that of the highly expressed genes of S.cerevisiae.


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