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Nucleic Acids Research, 1985, Vol. 13, No. 17 6317-6330
© 1985

Articles

The 31P-NMR specturm of the dodecamer d(GACGATATCGTC)

Johann Ott1, Fritz Ecksteikn1,* and Bernard A. Connolly2

1Max-Planck-Institut fur experimentelle Medizin, Abteilung Chemie Hermann-Rein-Strasse 3, D-3400 Gottingen, FRG 2Department of Biochemistry, University of Southampton Southampton SO9 3TU, UK

*To whom correspondence should be addressed

Received June 6, 1985. Accepted July 31, 1985.

The resonances in the 31P-NMR spectrum of the dodecamer d(GACGATATCGTC) have been assigned by regiospecific labelling with oxygen-17. All 11 resonances are clearly resolved at 26°C. Most noticeably, individual resonances for the dinucleoside phosphates d(CpG), d(TpC), d(GpA) and d(ApT) which occur more than once can clearly be distinguished. This indicates that the position of the phosphate group in the oligomer influences its 31P-NMB shift. This observation is in agreement with what has been found for the 31P-NMR spectra of d(CGCGAATTCGCG) [Ott, J. and Eckstein, F. (1985) Biochemistry 24] and d(GGAATTCC) [Connolly, B.A. and Eckstein, F. (1984) Biochemistry 23, 5523–5527]. In general, the chemical shift appears tha more at higher field the more central the dinucleoside phosphate is located in the oligomer. Exceptions are the resonances of dinucleoside phosphates of the type 5'-PyPu-3' which appear at lower field than expected from this rule. A reasonable correlation between 31P-NMR chemical shifts and the sum function of the base plane roll angles derived from Calladine's rule [Calladine, C.R. (1982) J. Mol. Biol. 161, 343–352] exists.


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