Skip Navigation

This Article
Right arrow Print PDF (1391K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kramerov, D.A.
Right arrow Articles by Georgiev, G.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kramerov, D.A.
Right arrow Articles by Georgiev, G.P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 18 6423-6437
© 1985

Articles

Nucleotide sequence of small polyadenylated B2 RNA

D.A. Kramerov, S.V. Tillib, A.P. Ryskov and G.P. Georgiev

Institute of Molecular Biology, USSR Academy of Sciences Vavilov str. 32, 117984 Moscow B-334, USSR

Received August 7, 1985. Accepted August 5, 1985.

Small poly(A)-containing RNA molecules which hybridize to the ubiquitous short repetitive sequence B2 and which are transcribed by RNA polymerase III have been identified in the cytoplasm of mouse cells. Here, we describe the structure of this small B2 RNA. A cDNA library was prepared from low-molecular- weight cytoplasmic poly(A)+RNA isolated from Ehrlich carcinoma cells and the clones which hybridized to B2 sequence were selected. The clones were sequenced and shown to contain B2 sequences followed by a poly(A) tract. The sequences of the cloned B2 RNAs different from each other by 3-10%, being similar in this respect to genomic B2 copies. Thus, B2 RNA is transcribed from many different B2 sequences in the genome. The 5'-ends of B2 RNA at least in most molecules coincide with the beginning of B2 genomic sequence. The poly(A) segments located at the 3'-end of small B2 RNA are the same size as in mRNA molecules , suggesting posttranscriptional formation. In some clones, additional sequences were detected between the 3'-end of B2 sequence and the poly(A) stretch . They seem to result from a lesion in the RNA polymerase III terminator in the corresponding B2 sequences. The possible significance of B2 sequences and small B2 ENA i s discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
A. J. Gentles, O. Kohany, and J. Jurka
Evolutionary Diversity and Potential Recombinogenic Role of Integration Targets of Non-LTR Retrotransposons
Mol. Biol. Evol., October 1, 2005; 22(10): 1983 - 1991.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Sinha, J. Gu, Y. Chen, and R. Reddy
Adenylation of Small RNAs in Human Cells. DEVELOPMENT OF A CELL-FREE SYSTEM FOR ACCURATE ADENYLATION ON THE 3'-END OF HUMAN SIGNAL RECOGNITION PARTICLE RNA
J. Biol. Chem., March 20, 1998; 273(12): 6853 - 6859.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.