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Nucleic Acids Research, 1985, Vol. 13, No. 18 6499-6513
© 1985

Articles

Human Immunoglobulin kappa light chain genes of subgroups II and III

H.-Gustav Klobeck, Alfons Meindl, Gabriele Combriato, Alan Solomon1 and Hans G. Zachau

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, FRG 1Department of Medicine, University of Tennessee, College of Medicine/Knoxville Unit Knoxville, TN 37920, USA

Received July 5, 1985. Accepted September 6, 1985.

The first complete sequences of functionally rearranged VK genes (abbreviations ref.l) of subgroups II and III are reported. The genes have been cloned from lyraphoid cell lines synthesizing KII or KIII light chains as evidenced from immunochemical analyses with anti-VK subgroup-specific antisera. These data, together with the sequence of a KIV gene (described in the accompanying paper) and those of previously published KI genes make possible a comparison of genes representative of the four known V region subgroups of human K light chains. The VKII gene is distinguished from the VKI, VKIII, and VKIV genes by a much longer intron within the leader sequence: 426 bp vs ca. 120–220 bp. Blot hybridization experiments with human DNA digests using probes from the KII and KIII genes and from the respective upstream regions help to define subgroup specific probes and hybridization conditions.


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