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Nucleic Acids Research, 1985, Vol. 13, No. 18 6577-6590
© 1985

Articles

Single stranded DNA binding proteins derive from hnRNP proteins by proteolysis in mammalian cells

Massimo Pandolfo, Omar Valentini, Giuseppe Biamonti, Carlo Morandi* and Silvano Riva+

Istituto di Genetica Biochimica ed Evoluzionistica C.N.R. Via Abbiategrasso 207 *Dipartimento di Genetica e Microbiologia, University of Pavia Via S. Epifanio 14, 27100 Pavia, Italy

+To whom correspondence should be addressed

Received June 10, 1985. Revised September 5, 1985. Accepted September 5, 1985.

As we have previously demonstrated, mammalian single stranded DNA binding proteins (ssDBP) and heterogeneous nuclear RNA binding proteins (hnRNP proteins) are antigenically and structurally related.

In this paper we show that ssDBP are specific proteolytic products of hnRNP core proteins. Proteolysis can be observed in crude extract, both total and nuclear and is not inhibited by the most commonly used protease inhibitors. Such phenomenon can be observed in HeLa cells, human fibroblasts and calf thymus extracts. A trypsin-like protease that cleaves purified hnRNP proteins to give ssDBP of Mr = 24–28 Kd can be purified from HeLa cells. A precursor-product relationship can be established between hnRNP core proteins type A and an ssDBP of 24 Kd (UP1).


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