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Nucleic Acids Research, 1985, Vol. 13, No. 18 6591-6604
© 1985

Articles

Structure of viral DNA in a rat cell line transformed by the cloned EcoRI-C fragment of adenovirus 12

Yutaka Kawarabayasi* and Hiroyuki Sugisaki

Institute for Chemical Research, Kyoto University Uji, Kyoto 611, Japan

*To whom correspondence should be addressed

Received May 29, 1985. Accepted August 23, 1985.

A DNA segment carrying viral DNA was cloned from a rat cell line transformed by the cloned EcoRI-C fragment(0 to 16.4 map units) of human adenovirus type 12(Adl2), and the viral sequence in the clone was analysed. The cloned segment contained the region from nucleotide positions 118 to 3520 of the Adl2 genome in the middle. No unique structure was found at the viral and non-viral DNA junctions. When examined the transforming activity, the conserved viral sequence was able to transform rat 3Y1 cells efficiently. Southern blotting analysis of the viral sequence in five re-transformed cell lines showed that the viral sequence was inserted at different sites of cellular DNA. These results indicate that (I) the Adl2 DNA moiety from the enhancer-promoter region of the E1A gene to the end of the E1B gene contains enough information for efficient transformation of the rat cell, and (II) integration of the viral sequence at unique cellular sites is not prerequisite for transformation.


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