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Nucleic Acids Research, 1985, Vol. 13, No. 18 6733-6743
© 1985

Articles

The 5'-flanking regions of three pea legumia genes: comparison of the DNA sequences

Grantley W. Lycett1,*, Ronald R. D. Croy, Anil H. Shirsat, D. Margaret Richards and Donald Boulter

Department of Botany, University of Durham South Road, Durham DH1 3LE, UK

*To whom correspondence should be addressed

Received June 17, 1985. Revised September 3, 1985. Accepted September 3, 1985.

Approximately 1200 nucleotides of sequence data from the promoter and 5'-flanking regions of each of three pea (Pisum sativum L.) legumin genes (legA, legB and legC) are presented. The promoter regions of all three genes were found to be identical including the ‘TATA box’, and ‘CAAT box’, and sequences showing homology to the SV40 enhancers. The legA sequence begins to diverge from the others about 300bp from the start codon, whereas the other two genes remain identical for another 550bp. The regions of partial homology exhibit deletions or insertions and some short, comparatively well conserved sequences. The significance of these features is discussed in terms of evolutionary mechanisms and their possible functional roles. The legC gene contains a region that may potentially form either of two mutually exclusive stem-loop structures, one of which has a stem 42bp long, which suggests that it could be fairly stable. We suggest that a mechanism of switching between such alternative structures may play some role in gene control or may represent the insertion of a transposable element.

1Present address: Department of Physiology and Environmental Science, University of Nottingham School of Agriculture, Sutton Bonington LE12 5RD, UK


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