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Nucleic Acids Research, 1985, Vol. 13, No. 18 6745-6752
© 1985

Articles

A new endonuclease recognizing the deoxynucleotide sequence C– CNNGG from the cyanobacterium Synechocystis 6701

Fabienne Calléja, Nicole Tandeau de Marsac*, Thérèse Coursin*, Hans van Ormondt and Adrian de Waard

Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden P.O. Box 9503, 2300 RA Leiden, The Netherlands *Unité de Physiologie Microbienne C. N. R. S. ERA 398, Départment de Biochimie et Génétique Moléculaire, Institut Pasteur, Rue du Dr. Roux 75724 Paris Cedex 15, France

Received June 17, 1985. Revised August 27, 1985. Accepted August 27, 1985.

A new sequence-specific endonudease from the cyanobacterium Synechocystis species PCC 6701 has been purified and characterized. This enzym, Seel, is unique in recognizing the nucleotide sequence:

Formula
and cleaves it at the position indicated by the symbol. Two other restriction endonucleases, Sec11 and SecIII, found in this organism are isoschizomers of MspI and Mstll, respectively.


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