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Nucleic Acids Research, 1985, Vol. 13, No. 19 6817-6832
© 1985

Articles

The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.)

J. Forde1,*, J-M. Malpica1,2, N.G. Halford1, P.R. Shewry1, O.D. Anderson3, F.C. Greene3 and B.J. Miflin1

1Rothamsted Experimental Station, Harpenden AL5 2JQ, UK 3USDA Western Regional Research Centre 800 Buchanan Street, Albany CA 94710, USA

*To whom correspondence should be addressed

Received August 23, 1985. Accepted September 12, 1985.

A cloned 8.2 kb EcoRI fragment has been isolated from a genomic library of DNA derived from Triticum aestivum L. cv. Cheyenne. This fragment contains sequences related to the high molecular weight (HMW) subunits of glutenin, proteins considered to be important in determining the elastic properties of gluten. The cloned HMW subunit gene appears to be derived from chromosome 1A. The nucleotide sequence of this gene has provided new information on the structure and evolution of the HMW subunits. However, hybrid-selection translation experiments suggest that this gene is silent.

2Present address: Dept. de Genetica, I.N.I.A. Madrid 35, Spain


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