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Nucleic Acids Research, 1985, Vol. 13, No. 19 6833-6846
© 1985

Articles

Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit

R.D. Thompson, D. Bartels and N.P. Harberd

Plant Breeding Institute, Maris Lane, Trumpington Cambridge CB2 2LQ, UK

Received August 23, 1985. Accepted September 12, 1985.

A high molecular weight glutenin gene in hexaploid wheat has been isolated by cloning in bacteriophage lambda and characterized. The gene correaponds to polypeptide 12 encoded by chromosome 1D in the variety "Chinese Spring". The coding sequence predicted contains seven cysteine residues six of which flank a central repetitive region comprising more than 70% of the polypeptide. These findings are related to the role of high molecular weight subunits in the viscoelastic theory of gluten structure.


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