Nucleic Acids Research, 1985, Vol. 13, No. 19 6867-6880
© 1985
Articles |
Cloning of eukaryotic protein synthesis initiation factor genes: isolation and characterization of cDNA clones encoding factor eIF-4A
1Biocenter, University of Basel CH-4056 Basel 2Hoffmann-La Roche Inc. CH-4002 Basel, Switzerland
3To whom reprint requests should be sent
Received July 25, 1985. Revised September 9, 1985. Accepted September 9, 1985.
Monoclonal antibodies directed against rabbit reticulocyte protein synthesis initiation factor 4A (eIF-4A) were used to isolate mouse cDNA clones expressing eIF-4A protein sequences in E.coli The identity of cDNA clones encoding eIF-4A sequences was confirmed by hybrid-selected translation and peptide mapping of the translation product. Analysis of the mRNA coding for eIF-4A from mouse liver and HeLa cells by Northern hybridization revealed two discrete mRNA species of approximately 2000 and 1600 nucleotides in length. The existence of two mRNAs in mouse and HeLa cells encoding eIF-4A was confirmed by cDNA sequencing.
4Present address: Max Planck Institut für Immunologie Stübeweg 51, D-7800 Freiburg, FRG
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