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Nucleic Acids Research, 1985, Vol. 13, No. 19 6999-7014
© 1985

Articles

Inhibition of HeLa cell DNA topoisomerase I by ATP and phosphate

Robert L. Low* and Joseph A. Holden

Departments of Pathology and Biological chemistry, Washington University School of Medicine St. Louis, MO 63110, USA

*To whom correspondence should be addressed

Received June 14, 1985. Revised September 9, 1985. Accepted September 11, 1985.

The relaxation activity of DNA topoisomerase I from HeLa cell nuclei is strongly inhibited by a variety of purine nucleotides in the presence but not absence of 1 mM potassium phosphate. For ATP, 3–4 mM causes nearly complete inhibition. The 2'-and 3'-AMP isomers are active as well in the presence of 1 mM phosphate, but the 5'-AMP isomer and adenosine are inert. At 3 mM ATP, the titration curve for phosphate is sigmoidal with inhibition beginning abruptly at about 0.5 mM. The negatively-supercoiled DNA isolated from an "inhibited" reaction is relaxed as well as the standard DNA template in the absence of ATP and phosphate suggesting that inhibition does not result from an alteration of the template which protects against its relaxation. Relaxation of positively-supercoiled DNA is also inhibited. Catalysis by E. coli DNA topoisomerase I and HeLa DNA topoisomerase II is not inhibited at concentrations of ATP and phosphate sufficient to cause 80–90% inhibition of HeLa type 1 enzyme.


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