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Nucleic Acids Research, 1985, Vol. 13, No. 19 7015-7024
© 1985

Articles

Interaction of the restriction endonuclease ScaI with its substrates

Keiko Kita, Nobutsugu Hiraoka, Fusao Kimizuka, Akira Obayashi, Hiroyuki Kojima*, Hideo Takahashi* and Hiuga Saito*

Central Research Laboratories, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu-shi, Shiga 520-21 *Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Received June 12, 1985. Revised September 9, 1985. Accepted September 16, 1985.

The kinetic conatants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- > deca-, dodeca-, hexadeca- > pBR322). The results were discussed with respect to the tertiary structure of substrate.


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