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Nucleic Acids Research, 1985, Vol. 13, No. 2 337-346
© 1985

Articles

Mammalian single-stranded DNA binding proteins and heterogeneous nuclear RNA proteins have common antigenic determinants

Omar Valentini, Giuseppe Biamonti, Massimo Pandolfo, Carlo Morandi1 and Silvano Riva*

Istituto di Genetica Biochimica ed Evoluzionistica CNR, Via Abbiategrasso, 207 1Dipartimento di Genetica e Microbiologia, University of Pavia Via S. Epifanio, 14, 27100 Pavia, Italy

*To whom correspondence should be addressed

Received November 28, 1984. Accepted January 3, 1985.

Antibodies were raised in rabbit against a pure subset of calf thymus single-stranded DNA binding proteins (ssDBPs) and purified by affinity chromatography on antigen-Sepharose. In Western blot experiments these antibodies were shown to react to the same extent with the whole family of bovine ssDBPs, as well as with ssDBPs from HeLa cells. When used to stain total cell extracts from both calf thymus and HeLa cells the antibodies reacted only with bands corresponding to the ssDBPs and with a set of bands of higher molecular weight, whose electrophoretic pattern matched that of the 40S hnRNP core proteins. In effect we observed that purified 40S hnRNP core proteins from HeLa cells were strongly reactive with the antibodies. Moreover after partial tryptic digestion HeLa cells ssDBPs and hnRNPs produced immunoreactive fragments of the same molecular weight and isoelectric point. Extensive structural homologies can thus be evidenced between these two classes of proteins, which share the property of selective binding to single-stranded nucleic acids.


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