Skip Navigation

This Article
Right arrow Print PDF (1925K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kwok, W. W.
Right arrow Articles by Nester, E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kwok, W. W.
Right arrow Articles by Nester, E. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 2 459-471
© 1985

Articles

Unusual plasmid DNA organization in an octopine crown gall tumor

William W. Kwok1 and Eugene W. Nester+

Department of Biochemistry, University of Washington Seattle, WA 98195, USA +Department of Microbiology and Immunology, University of Washington Seattle, WA 98195, USA

Received September 21, 1984. Revised December 11, 1984. Accepted December 11, 1984.

A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization studies and genomic cloning indicated that some segments of the T-DNA have undergone deletions. One of the clones contained two copies of T-DNA which were inverted in orientation with respect to each other.

Two left ends of TL DNA from the 1595501 tumor line and the corresponding region of the octopine plasmid were sequenced. Comparison of the various cloned T-DNA sequences with Ti-plasmid sequence indicated that while there is an association with a 25 base pair direct repeat, there is no specific set of base pairs in the T-DNA at which divergence from Ti-plasmid sequences occurred.

1Present address: Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Kohli, M. Leech, P. Vain, D. A. Laurie, and P. Christou
Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots
PNAS, June 9, 1998; 95(12): 7203 - 7208.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
G Gheysen, R Villarroel, and M Van Montagu
Illegitimate recombination in plants: a model for T-DNA integration.
Genes & Dev., February 1, 1991; 5(2): 287 - 297.
[Abstract] [PDF]

Home page
 ScienceHome page
K. WANG, S. E. STACHEL, B. TIMMERMAN, M. VAN MONTAGU, and P. C. ZAMBRYSKI
Site-Specific Nick in the T-DNA Border Sequence as a Result of Agrobacterium vir Gene Expression
Science, January 30, 1987; 235(4788): 587 - 591.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.