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Nucleic Acids Research, 1985, Vol. 13, No. 2 573-584
© 1985

Articles

Methylation of thymine residues during oligonucleotide synthesis

X. Gao, B.L. Gaffney, M. Senior, R.R. Riddle and R.A. Jones

Department of Chemistry, Rutgers, The State University of New Jersey New Brunswick, NJ 08903,USA

Received December 3, 1984. Accepted January 3, 1985.

Thyinine residues in an oligodeoxyribonucleotide are subject to methylation at N3 by the internucleotide methyl phosphotriester linkages. This alkylation occurs most rapidly in the presence of a strong base such as DBU, but also takes place, at a much slower rate, during oligonucleotide synthesis.


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